Background. Nosocomial infections occur among patients during their stay in hospitals. The severity of infection depends on the\ncharacteristics of microorganisms with a high risk of being acquired when the environment is contaminated. Antibiotic-resistant\nbacteria are emerging rapidly around the globe creating a serious threat. Methods. A cross-sectional study was conducted from\nDecember 2016â??February 2017 at Mizan-Tepi University Teaching Hospital, Southwest Ethiopia. Samples were collected from the\nequipment and hospital surfaces. The isolated bacteria were checked for susceptibility by the Kirbyâ??Bauer disc diffusion method\nfollowing the standards of CLSI 2014. Health professionals and sanitary team members were included in the study which assessed\nthe disinfection practice of objects from which samples were taken. Data were analyzed using SPSS version 20.0. Results. A total of\n201 swab samples were taken, and most bacteria were recovered from thermometer and floor consisting of 21.6% S. aureus, 19.3%\nCoNS, 15.9% E. coli, 14.8% Klebsiella species, 11.4% P. aeruginosa, 10.2% Proteus species, and 6.8% Serratia species. The most\nmultidrug resistant organisms were S. aureus (79%), Klebsiella species (53.8%), CoNS (47%), and Proteus species (44.4%). Only\n6.45% of health professionals disinfect their stethoscope consistently. Conclusion. S. aureus, CoNS, and E. coli were the predominant\nisolates. Most isolates showed highest susceptibility to ciprofloxacin and least to ampicillin and penicillin. There is no\nregular sanitation and disinfection of hospital equipment and surfaces.
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